Journal: Cell reports

Article Title: A Purine Nucleotide Biosynthesis Enzyme Guanosine Monophosphate Reductase is a Suppressor of Melanoma Invasion

doi: 10.1016/j.celrep.2013.09.015

Figure Lengend Snippet: (A) Schematic representation of the de novo purine biosynthesis and salvage pathway. Enzymes and their products are shown by ovals and open boxes, respectively. * IMPDH 1/2 are rate-limiting enzymes and MPA targets. (B) Total cellular extracts from independently isolated populations of normal human melanocytes (NHM) and indicated melanoma cell lines were probed in western blotting with indicated antibodies. (C) Expression of GMPR and IMPDH2 in thin, thick primary melanomas and melanoma metastases. The box plots represent the distribution of the IHC index. The median, first quartile, and third quartile are shown in the “box” with outlying samples represented by points. The dashed lines represent the interquartile range (IQR) times 1.5 added to the first and third quartiles. The number of patient samples (n) is indicated for each cohort. (D) Representative IHC images for GMPR and IMPDH2 from the data presented in C. See also Figure S1.

Article Snippet: For IMPDH2 antibodies (Atlas Antibodies, HPA001400) and GMPR antibodies (Sigma-Aldrich HPA021476), the Novocastra (Newcastle,UK) PowerVision kit was used for visualization, followed by Fast Red (Thermo Scientific, Wilmington, DE).

Techniques: Isolation, Western Blot, Expressing